Fractionation / Extraction Kit

RIPA buffer

WSE-7420 EzRIPA Lysis kit



Purpose and Application

  • Whole cell extract preparation for the following applications
     Electrophoresis/ Western blotting/ Immunoprecipitation/ ELISA/ Enzyme activity/ etc.


  • Sample preparation for electrophoresis, immunoprecipitation, ELISA, enzyme activity experiments, etc.
  • Standard set includes Protease inhibitor and Phosphatase inhibitor.
  • Since EzRIPA Lysis kit is amine-free buffer that based on HEPES buffer, labeling experiments of prepared sample can be applied directly.


WSE-7420 EzRIPA Lysis kit
Applicable amount 100 samples, up to 2 x 10⁷ cell/sample
Storage 1 year
Package – EzRIPA Lysis buffer: 100 mL
(20 mM HEPES pH 7.5, 1% IGEPAL® CA-630*, 0.1% SDS, 0.5% Deoxycholic acid, 150 mM Sodium Chloride)
– Protease Inhibitor (100x): 1 mL
(Aprotinin, Pepstatin A, Leupeptin)
– Phosphatase Inhibitor (100X): 10 mL
(Sodium fluoride, Sodium orthovanadate (V), β-sodium glycerophosphate)
* IGEPAL® CA-630 is the same as NonidetP-40, and a trade mark registration of RhonePoulenc AG Co.


Ordering Information

Code No. Description Unit
2332336 WSE-7420 EzRIPA Lysis kit 1 pk